High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
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چکیده
منابع مشابه
High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Microbes in soils and other environments produce extracellular enzymes to depolymerize and hydrolyze organic macromolecules so that they can be assimilated for energy and nutrients. Measuring soil microbial enzyme activity is crucial in understanding soil ecosystem functional dynamics. The general concept of the fluorescence enzyme assay is that synthetic C-, N-, or P-rich substrates bound with...
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Changes in the spectrum and rates of extracellular enzyme activities in seawater following aggregate formation K. Ziervogel, A. D. Steen, and C. Arnosti Department of Marine Sciences, University of North Carolina at Chapel Hill, Chapman Hall, CB#3300, Chapel Hill, NC 27599-3300, USA now at: Center for Geomicrobiology, University of Århus, Ny Munkgade 1540, 0800 Århus C, Denmark Received: 16 Nov...
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23 Chlamydia trachomatis is an obligate intracellular mucosotropic pathogen that causes human 24 infections of global importance. C. trachomatis causes trachoma, the leading cause of 25 preventable blindness worldwide and is the most common cause of bacterial sexually transmitted 26 disease. Although oculogenital infections are treatable with antibiotics, a vaccine is needed to 27 control C. tr...
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Microbial extracellular hydrolytic enzymes that degrade organic matter in aquatic ecosystems play key roles in the biogeochemical carbon cycle. To provide linkages between hydrolytic enzyme activities and genomic or metabolomic studies in aquatic environments, reliable measurements are required for many samples at one time. Extracellular proteases are one of the most important classes of enzyme...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2013
ISSN: 1940-087X
DOI: 10.3791/50961